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基本信息姓名:赵剑出生年月:1968.7
性别:男硕/博导:博导
民族:汉开设课程:基础遗传学
职称:教授研究方向:作物代谢组学,大豆作物遗传育种与改良 ,植物次生代谢和代谢工程,植物特殊营养物和天然药物的生物合成
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学位:生药学博士
联系方式办公电话:027-87385199
电子邮件:jianzhao@mail.hzau.edu.cn
个人简介赵剑,男 ,1968年生于内蒙古,博士 、教授、博士生导师.1992年内蒙古师范大学生物系获生物学学士学位,1995年东北师范大学获植物学硕士学位.1999年中国医学科学院/中国协和医科大学药物研究所获生药学博士学位.2000-2002年获得日本学术振兴会资助奖励在日本九州大学农学部做抗真菌单萜Thujaplicin的生物合成和生产的研究.2002-2012年先后在美国堪萨斯州立大学生物化学系、贝勒医学院/美国农业部儿童营养研究中心(BaylorCollegeofMedicine/USDA/ARSChildren’sNutritionResearchCenter)和TheSamuelRobertsNobleFoundation等大学和研究机构学习和工作。长期从事植物天然化合物的生物合成和代谢工程,脂质代谢等领域的研究 。在植物次生代谢和有用天然产物的诱导合成 、细胞生物工程生产、生物合成及其调节的分子生物学机理等方面做了大量前沿性研究。近几年来以豆科模式植物Medicagotruncatula为研究材料在黄酮类转运和合成调控研究方面 ,尤其是缩合丹宁和花色素苷的细胞内转运及其合成的转录调节机理方面获得了重大发现并申请了专利,现在其实验室专注于大豆黄酮类化合物,多萜类 ,油脂等特殊营养物质的生物合成和相关大豆种子品质育种和遗传改良的研究。将豆科模式植物Medicagotruncatula的转录组学和代谢组学研究模式应用到大豆品质的功能基因组学中,用代谢组学和转录组学的技术阐明它们的生物合成和调节的分子机理并把获得的新知识和新手段应用到大豆分子育种和传统育种 。
国际学术组织兼职和服务:
从2007年起担任国际期刊RecentPatentsonBiotechnology(byBenthamScience)的编委;为ThePlantJournal,PlantPhysiology,PlantCell&Environment,Planta,AnnalsofBotany,NewPhytologist,BiotechnologyandBioengineering,AppliedMicrobiologyandBiotechnology;PlantCellReports;BiotechnologyProgress;PhytochemistryReviews;ProcessBiochemistry;BiotechnologyandAppliedBiochemistry等国际学术期刊的邀请审稿人
科研项目华中农业大学和作物遗传育种与改良国家重点实验室的科研启动费。
科技部973项目—水稻和玉米等作物代谢谱数据库的建立和利用
发明专利及获奖情况主要奖:
2000:日本学术振兴会(JapanSocietyforthePromotionofScience)外国人特别研究员奖及研究经费
专利:VerdierJA,ZhaoJ,DixonRA,UdvardiMK.MethodsandCompositionsforRegulatingProducingProanthocyanidins.PendingapplicationtoUSApatentoffice.
发表的论文及著作学术专著(章节):
1.ZhaoJ,SakaiK.(2001)Involvementofperoxidasesandhydrogenperoxideinthemetabolismof-thujaplicininfungalelicitor-treatedCupressuslusitanicasuspensioncultures.In:MorohoshiN,KomamineA,(eds),MolecularBreedingofWoodyPlants:ProceedingsoftheInternationalWoodBiotechnologySymposium.ProgressinBiotechnology,Vol18,Elsevier:Tokyo,p263-272.
2.ZhaoJ.(2006)Elicitorsignaltransductionleadingtobiosynthesisofplantdefensivesecondarymetabolites.In:TeixeiradaSilvaJ(eds),Floriculture,OrnamentalandPlantBiotechnology.Vol3.GlobalScienceBooksLtd:Tokyo,London.p344-357.
3.ZhaoJ.(2011)Phytonutrientandphytotherapyforimprovinghealth.In:PeterPrendergastandMelvinA.Shiffman(eds),AestheticMedicine:ArtandTechniques.Springer-Verlag:Heidelberg,Germany,Part2,p47-58.
4.ZhaoJ.(2012)Nutraceuticals/functionalfoodsforimprovinghealthandpreventingdisease.In:OliverKayserandHeribertWarzecha(eds),PharmaceuticalBiotechnology:DrugDiscoveryandClinicalApplications,2ndEdition.Wiley-VCHVerlag:Weinheim,Germany.p599-628
期刊论文(JCR-2010,Googlescholarcitation-2015)
ZhaoJ,LiP,MotesCM,ParkS,HirschiKD.(2015)CHX14isaplasmamembraneK-effluxtransporterthatregulatesK+redistributioninArabidopsisthaliana.PlantCell&Environmentinpress
ZhaoJ.(2015)PhospholipaseDandphosphatidicacidinplantdefenceresponse:fromprotein–proteinandlipid–proteininteractionstohormonesignaling.Journalofexperimentalbotany.Inpress
http://jxb.oxfordjournals.org/content/early/2015/02/12/jxb.eru540.full.pdf.
5.ZhaoJ*,WangX.(2013)BiochemicalanalysisoftheinteractionbetweenphospholipaseDα1andGTP-bindingproteinα-subunitfromArabidopsisthaliana.MethodsinMolecularBiology1043:21-35.*通讯作者
6.ZhaoJ*,DevaiahSP,WangC,WeltiR,WangX*.(2013)ArabidopsisphospholipaseDβ1modulatesdefenseresponsestobacterialandfungalpathogens.NewPhytologist199:228-240*共同通讯作者.杂志影响因子6.6被引用次数:16
7.VerdierJ#,ZhaoJ#,Torres-JerezI,GeS,HeXZ,MysoreKS,DixonRA,UdvardiMK(2012)TheMtPARMYBtranscriptionfactoractsasanon-switchforproanthocyanidinbiosynthesisinMedicagotruncatula.ProcNatlAcadSciUSA109:1766-1771杂志影响因子9.7;#共同第一作者.被引用次数:34
8.ZhaoJ,WangC,BedairM,WeltiR,SumnerLW,IvanBaxterI,WangX.(2011)SuppressionofphospholipaseDγsconfersanincreasedaluminumresistanceinArabidopsisthaliana.PLoSOne6(12):e28086杂志影响因子4.4;被引用次数:15
9.ZhaoJ,HuhmanD,ShadleG,HeXZ,SumnerLW,TangY,DixonRA.(2011)MATE2mediatesvacuolarsequestrationofglycosylatedandmalonylatedflavonoidsinMedicagotruncatula.PlantCell23:1536-1555.杂志影响因子9.3;被引用次数:48
10.ZhaoJ,DixonRA(2010)The“ins ”and“outs”offlavonoidtransport.TrendsinPlantScience15:72-80.杂志影响因子10.0;被引用次数:102
11.ZhaoJ,PangYZ,DixonRA(2010)Themysteriesofproanthocyanidintransportandpolymerization.PlantPhysiology153:437-443杂志影响因子6.4;被引用次数:80
12.ZhaoJ,ConnortonJM,GuoYQ,LiX,ShigakiT,HirschiKD,PittmanJK.(2009)FunctionalstudiesofsplitArabidopsisCa2+/H+exchangers.JournalofBiologicalChemistry284:34075-34083.杂志影响因子5.3;被引用次数:30
13.ZhaoJ,Dixon,RA.(2009)MATEtransportersfacilitatevacuolaruptakeofepicatechin3'-O-glucosideforproanthocyanidinbiosynthesisinMedicagotruncatulaandArabidopsis.PlantCell21:2323-2340.杂志影响因子9.3;被引用次数:108
14.MeiH,ChengNH,ZhaoJ,ParkS,EscarenoRA,PittmanJK,HirschiKD.(2009)RootdevelopmentundermetalstressinArabidopsisthalianarequirestheH+/cationantiporterCAX4.NewPhytologist183:95-105.杂志影响因子6.5;被引用次数:28
15.ZhaoJ,ShigakiT,MeiH,GuoYQ,ChengNH,,HirschiKD.(2009)InteractionbetweenArabidopsisCa2+/H+exchangersCAX1andCAX3.JournalofBiologicalChemistry284:4605-4615.杂志影响因子5.3;被引用次数:28
16.ZhaoJ,ChengNH,MotesCM,BlancaflorEB,MooreM,GonzalesN,PadmanabanS,SzeH,WardJM,HirschiKD.(2008)AtCHX13isaplasmamembraneK+transporter.PlantPhysiology148:796-807.杂志影响因子6.4;被引用42次
17.ZhaoJ,BarklaBJ,MarshallJ,PittmanJK,HirschiKD.(2008)TheArabidopsiscax3mutantsdisplayalteredsalttolerance,pHsensitivityandreducedplasmamembraneH+-ATPaseactivity.Planta227:659-669.杂志影响因子3.08;被引用次数:63
18.MeiH,ZhaoJ,PittmanJK,LachmansinghJ,ParkS,HirschiKD.(2007)InplantaregulationoftheArabidopsisCa2+/H+antiporterCAX1.JournalofExperimentalBotany58:3419-3427.杂志影响因子4.8;被引用次数:43
19.ZhaoJ.(2007)Interplayamongnitricoxideandreactiveoxygenspecies:Acomplexnetworkdeterminingcellsurvivalordeath.PlantSignaling&Behavior2:544-547(invitedAddendumtoNewPhytologist175:215-229).杂志影响因子0;被引用次数:17通讯作者
20.ZhaoJ.FujitaK,SakaiK.(2007)Reactiveoxygenspecies,nitricoxide,andtheirinteractionsplaydifferentrolesinCupressuslusitanicacelldeathandphytoalexinproduction.NewPhytologist175:215-229.杂志影响因子6.5;被引用次数:30通讯作者
21.ZhaoJ.(2007)Nutraceutical,nutritionaltherapy,phytonutrients,andphytotherapyforimprovementofhumanhealth:Aperspectiveonplantbiotechnologyapplication.RecentPatentsonBiotechnology1:75-97(invitedreview).被引用次数:52通讯作者
22.ZhaoJ(2007)Planttroponoids:Chemistry,biologicalactivity,andbiosynthesis.CurrentMedicinalChemistry14(24):2597-2621.杂志影响因子4.6;被引用次数:28通讯作者
23.ZhaoJ,VerpoorteR.(2007)ManipulatingindolealkaloidproductioninCatharanthusroseuscellculturesinbioreactors:frombiochemicalprocessingtometabolicengineering.PhytochemistryReviews6:435-457(Invitedreview)杂志影响因子3.1;被引用次数:73通讯作者
24.BusemanCM,TamuraP,SparksAA,BaughmanE,MbbttaS,ZhaoJ,RothMR,EschSW,ShahJ,WilliamsTD,WeltiR.(2006)Woundingstimulatestheaccumulationofglycerolipidscontainingoxophytodienoicacidanddinor-oxophytodienoicacidinArabidopsisleaves.PlantPhysiology142:28-39.杂志影响因子6.4;被引用次数:115
25.MishraG,ZhangW,DengF,ZhaoJ,WangX(2006)AbifurcatingpathwaydirectsabscisicacideffectsonstomatalclosureandopeninginArabidopsis.Science312:264-266.杂志影响因子31;被引用次数:283
26.ZhaoJ,MatsimagaY,FujitaK,SakaiK.(2006)Metabolicfluxandsignalanalysisofb-thujaplicinandmonoterpenebiosynthesisinelicitedC.lusitanicacellculturebyelicitorandmethyljasmonate.MetabolicEngineering8:14-29杂志影响因子5.5;被引用次数:17通讯作者
27.ShigakiT,BarkalaB,Miranda-VergaraMC,ZhaoJ,PantojaO,HirschiK(2005).IdentificationofacriticalhistidineinvolvedinmetaltransportactivityinArabidopsiscation/HexchangerCAX1.JournalofBiologicalChemistry280:30136-30142杂志影响因子5.3;被引用次数:42
28.ZhaoJ,FujitaK,SakaiK.(2005)Oxidativeburstinplantcellcultureprocess:aroleinproductionof β-thujaplicinbyCupressuslusitanicasuspensionculture.BiotechnologyandBioengineering90:621-631.杂志影响因子3.7;被引用次数:38通讯作者
29.ZhaoJ,DavisLC,VerpoorteR(2005)Elicitorsignaltransductionleadingtoproductionofplantsecondarymetabolites.BiotechnologyAdvances23:283-333.杂志影响因子5.3;被引用次数:789通讯作者
30.ZhangW,QinC,ZhaoJ,WangX.(2004)PhospholipaseDα1-derivedphosphatidicacidinteractswithABI1phosphatase2Candregulatesabscisicacidsignaling,ProcNatlAcadSciUSA101:9508-9513.杂志影响因子9.7;被引用次数:299
31.ZhaoJ,ZhengSH,FujitaK,SakaiK.(2004)JasmonateandethylenesignalingpathwaysandtheirinteractionareintegralpartsoftheelicitorsignaltransductionleadingtophytoalexinbiosynthesisinCupressuslusitanicacellcultures.JournalofExperimentalBotany55:1003-1012.杂志影响因子4.8;被引用次数:41通讯作者
32.ZhaoJ,GuoY,KosaihiraA,SakaiK.(2004)Rapidmetabolismofphosphoinositidesanditspossiblerolesinphytoalexinbiosynthesisinelicitor-treatedCupressuslusitanicacellcultures.Planta219:121-131.杂志影响因子3.0;被引用次数:24通讯作者
33.ZhaoJ,GuoY,FujitaK,SakaiK.(2004)InvolvementofcAMPsignalinginelicitor-inducedphytoalexinaccumulationinCupressuslusitanicacellcultures.NewPhytologist161:723-733.杂志影响因子6.5;被引用次数:31
34.ZhaoJ,WangX.(2004).ArabidopsisphospholpaseD,PLDα1interactswithArabidopsisheterotrimericG-proteinα-subunitthroughamotifanalogoustotheDRYmotifinG-protein-coupledreceptors.JournalofBiologicalChemistry279:1794-1800.杂志影响因子5.3;被引用次数:131
35.ZhaoJ,SakaiK.(2003)Multiplesignalingpathwaysmediatefungalelicitor–induced-thujaplicinaccumulationinCupressuslusitanicacellcultures.JournalofExperimentalBotany54:647-656.杂志影响因子4.8;被引用次数:74
36.ZhaoJ,SakaiK.(2003)Peroxidasesareinvolvedinbiosynthesisandmetabolismof-thujaplicininfungalelicitor-treatedCupressuslusitanicasuspensioncultures.NewPhytologist159:719-731.杂志影响因子6.5;被引用次数:18
37.ZhaoJ,FujitaK,YamadaJ,SakaiK.(2001)Improved-thujaplicinproductioninCupressuslusitanicasuspensionculturesbyfungalelicitorandmethyljasmonate.AppliedMicrobiologyandBiotechnology55:301-305.杂志影响因子3.2;被引用次数:49
38.ZhaoJ,HuQ,GuoYQ,ZhuWH.(2001)Effectsofstressfactors,bioregulatorsandprecursorsonindolealkaloidproductioninthecompactcallusclusterscultureofCatharanthusroseus.AppliedMicrobiologyandBiotechnology55:693-698.杂志影响因子3.2;被引用次数:43通讯作者
39.ZhaoJ,HuQ,GuoYQ,ZhuWH.(2001)Elicitor-inducedindolealkaloidbiosynthesisinCatharanthusroseuscellculturesisrelatedtoCa2+-influxandtheoxidativeburst.PlantScience161:423-431.杂志影响因子2.4;被引用次数:73通讯作者
40.ZhaoJ,HuQ,ZhuWH.(2001)EnhancedcatharanthineproductioninCatharanthusroseuscellculturesbycombinedelicitortreatmentinshakeflasksandbioreactors.EnzymeandMicrobialTechnology28:673-681.杂志影响因子2.9;被引用次数:107通讯作者
41.ZhaoJ,ZhuWH ,HuQ(2001)SelectionoffungalelicitorstoincreaseindolealkaloidaccumulationinCatharanthusroseussuspensioncellculture.EnzymeandMicrobialTechnology28:666-672.杂志影响因子2.9;被引用次数:62通讯作者
42.ZhaoJ,Wei-HuaZhu,WH,HuQ(2001)EffectsoflightandplantgrowthregulatorsonthebiosynthesisofvindolineandotherindolealkaloidsinCatharanthusroseuscalluscultures.PlantGrowthRegulation33:43–49杂志影响因子1.6;被引用次数:43通讯作者
43.ZhaoJ,ZhuWH,HuQ(2000)EnhancedajmalicineproductioninCatharanthusroseuscellculturesbycombinedelicitortreatment:fromshake-flaskto20-lairliftbioreactor.BiotechnologyLetters22:509-514.杂志影响因子1.7;被引用次数:37通讯作者
部分中文期刊论文:
1.赵剑,朱蔚华,吴蕴褀 ,胡秋(1999)反相高效液相色谱法测定长春花组织和细胞培养物中吲哚生物碱的含量。药学学报.7:
2.赵剑,朱蔚华,吴蕴褀 ,胡秋(1999)长春花叶片愈伤组织诱导过程中吲哚生物碱的合成模式和含量的变化及相关酶活性的调节。中草药.1999,7
3.赵剑,杨文杰,朱蔚华(1999)细胞色素P450和植物的次生代谢 。生命科学 ,1999,2(综合译述)
4.赵剑,朱蔚华(1998)几种红豆杉植物及其愈伤组织的几种同工酶比较研究。中草药.29(6):402-405
5.赵剑 ,朱蔚华,吴蕴褀(1998)红豆杉组织培养中sinenxans高产细胞系TS-19的同工酶的动态研究。西北植物学报.18(3):339-347
6.赵剑,朱蔚华 ,方起程(1997)紫杉醇及其相关化合物的生物合成和生物工程的研究进展 。中草药.28(增刊):28-32(综合译述)<